![]() ![]() ![]() Transformed data was exported using the Kaluza Cytobank Plugin. Data were acquired on a 13 detector / 3 laser CytoFLEX V5-B5-R3 (PN: C09734) compensation and logicle transformation were adjusted using Kaluza Analysis software. Lineage markers and cytokine expression were assessed after six hours with a 13-color antibody cocktail described below (Table 1). To generate the data used in this Application Note, cryopreserved human peripheral blood mononuclear cells (PBMC) from healthy donors were thawed and rested overnight for cell function recovery stimulated using Dynabead T-cell Activator anti-CD3/CD28 (ThermoFisher Scientific) in presence of protein transport inhibitor (Brefeldin A). How to use heatmaps to help you identify the phenotype of a specific cluster/metacluster. ![]()
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